_:vb140785 "GOC:pt" . _:vb140785 "2018-05-01" . _:vb140785 "http://orcid.org/0000-0002-1706-4196" . _:vb140785 . _:vb140785 "http://www.wormbase.org" . _:vb140785 "\"To test if our purified TFIIH and pol II were transcriptionally active, we first combined both epitope-tagged multiprotein complexes with bacterially expressed recombinant (r) human TFIIB, rTBP, rTFIIE, rTFIIF, a supercoiled DNA template (pG5MLT, which contains five Gal4-binding sites preceding the adenovirus major late core promoter elements), and a linearized DNA template (pML\u039453, which contains the adenovirus major late core promoter elements without the Gal4-binding sites). As shown in Fig.5 A, both immunoaffinity-purified pol II complexes were equally active in transcribing the DNA templates, in conjunction with f:TFIIH, purified from the ammonium sulfate fraction, and other GTFs (comparelane 1 with lane 10).\" PMID:9852112" . _:vb140785 . _:vb140785 . _:vb140785 . _:vb140785 .