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Namespace Prefixes

Prefix	IRI
n8	http://purl.obolibrary.org/obo/
n5	http://model.geneontology.org/5323da1800000002/
dc	http://purl.org/dc/elements/1.1/
n9	http://purl.obolibrary.org/obo/#
rdfs	http://www.w3.org/2000/01/rdf-schema#
rdf	http://www.w3.org/1999/02/22-rdf-syntax-ns#
owl	http://www.w3.org/2002/07/owl#
xsdh	http://www.w3.org/2001/XMLSchema#
n6	http://purl.org/pav/
n4	http://geneontology.org/lego/

Statements

Subject Item

_:vb140784

rdf:type

owl:Axiom

rdfs:comment

"Dramatically reduced UV-dependent ubiquitylation was indeed observed in the stable Nedd4 knockdown cells compared to wild-type cells immediately after UV irradiation, but after 20 min, the level of ubiquitylation in the two cell lines was already similar, as expected (Figure 5D, compare lanes 5 and 6, and 7 and 8)." PMID:17996703 "To investigate whether they could support human RNAPII ubiquitylation in vitro, highly purified human RNAPII was used as substrate, with human Uba1 and UbcH7 (or UbcH5C) involved as E1 and E2, respectively (Figure 6C, lanes 7–10). In this highly purified, reconstituted human RNAPII ubiquitylation system (see Figure S4 for purity of these proteins), Nedd4 (and Smurf1), but none of the other Rsp5 homologs, supported robust ubiquitylation of RNAPII (Figure 6C, lanes 2 and 3)." PMID:17996703 "Several independent experiments showed that UV-induced ubiquitylation was reproducibly affected only by Nedd4 siRNA (Figure 5C, compare lane 4 with lane 14 [control] and other knockdowns; data not shown). This indicates that Nedd4 is required for UV-induced ubiquitylation of RNAPII in vivo." PMID:17996703

dc:date

2018-05-01

dc:contributor

http://orcid.org/0000-0002-1706-4196

owl:annotatedProperty

n8:RO_0002333

owl:annotatedSource

n5:5323da180000003

owl:annotatedTarget

n9:5323da1800000002%2F5595c4cb00000122

n6:providedBy

http://www.wormbase.org

n4:evidence

n5:5ae3b0f600000042 n5:5ae3b0f600000039