"RNAPII from chromatin was immunoprecipitated, and the association of the six Rsp5 homologs was tested by western blotting (Figures 7B–7G). Nedd4, but none of the other Rsp5 homologs, coimmunoprecipitated with RNAPII from chromatin, and importantly, more of this protein was associated with the polymerase after UV irradiation (Figures 7B–7G, compare lanes 1 and 2 with 5 and 6)." PMID:17996703